EMPIAR-10727 - Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM [multiple data sets in MRC format]

EMPIAR-10727

Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM [multiple data sets in MRC format]

Publication:

Locating macromolecular assemblies in cells by 2D template matching with cisTEM

Lucas BA, Himes BA, Xue L, Grant T, Mahamid J, Grigorieff N

eLife 10 (2021)

PMID: 34114559

DOI: 10.7554/elife.68946

Related EMDB entry:

EMD-23772 EMD-23771

Deposited:

2021-04-04

Released:

2021-07-07

Last modified:

2021-07-21

Imageset size:

10.99 GB

Imageset DOI:

10.6019/EMPIAR-10727

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
picked particles - single frame - unprocessed
picked particles - single frame - processed

Image Sets

1. Exposure-weighted micrographs of M. pneumoniae

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

220

Image size:

(4032, 2864)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.5, 1.5)

Details:

Particles were selected by 2D template matching with high-resolution templates of either M. pneumoniae or B. subtilis 50S subunits, and using a threshold with an expected false positive rate of 1 per image.

Files:

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Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

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Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Particle stack and cisTEM star file for 50S ribosomal subunits identified in the associated 220 images using the M. pneumoniae 50S template

Category:

picked particles - single frame - unprocessed

Image format:

MRC

No. of images or tilt series:

5080

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.5, 1.5)

Details:

Particle stack and cisTEM star file of 50S ribosomal subunits identified in the associated 220 images of M. pneumoniae, using a M. pneumoniae 50S template

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Particle stack and cisTEM star file for 50S ribosomal subunits identified in the associated 220 images using the B. subtilis 50S template

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

1172

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.5, 1.5)

Details:

Particle stack and cisTEM star file of 50S ribosomal subunits identified in the associated 220 images of M. pneumoniae, using a B. subtilis 50S template

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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