EMPIAR-10727 - Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM [multiple data sets in MRC format]

EMPIAR-10727

Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM [multiple data sets in MRC format]

Publication:

Locating macromolecular assemblies in cells by 2D template matching with cisTEM

Lucas BA, Himes BA, Xue L, Grant T, Mahamid J, Grigorieff N

eLife 10 (2021)

PMID: 34114559

DOI: 10.7554/elife.68946

Related EMDB entry:

EMD-23772 EMD-23771

Deposited:

2021-04-04

Released:

2021-07-07

Last modified:

2021-07-21

Imageset size:

10.99 GB

Imageset DOI:

10.6019/EMPIAR-10727

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
picked particles - single frame - unprocessed
picked particles - single frame - processed

Image Sets

1. Exposure-weighted micrographs of M. pneumoniae

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

220

Image size:

(4032, 2864)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.5, 1.5)

Details:

Particles were selected by 2D template matching with high-resolution templates of either M. pneumoniae or B. subtilis 50S subunits, and using a threshold with an expected false positive rate of 1 per image.

Files:

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Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Particle stack and cisTEM star file for 50S ribosomal subunits identified in the associated 220 images using the M. pneumoniae 50S template

Category:

picked particles - single frame - unprocessed

Image format:

MRC

No. of images or tilt series:

5080

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.5, 1.5)

Details:

Particle stack and cisTEM star file of 50S ribosomal subunits identified in the associated 220 images of M. pneumoniae, using a M. pneumoniae 50S template

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Particle stack and cisTEM star file for 50S ribosomal subunits identified in the associated 220 images using the B. subtilis 50S template

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

1172

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.5, 1.5)

Details:

Particle stack and cisTEM star file of 50S ribosomal subunits identified in the associated 220 images of M. pneumoniae, using a B. subtilis 50S template

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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