EMPIAR-10596 - Human DPP9-CARD8 complex-VbP [multiple data sets in TIFF format]

EMPIAR-10596

Human DPP9-CARD8 complex-VbP [multiple data sets in TIFF format]

Publication:

Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment

Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H

Immunity 54 (2021) 1392-1404.e10

PMID: 34019797

DOI: 10.1016/j.immuni.2021.04.024

Related EMDB entry:

EMD-22402

Deposited:

2020-12-13

Released:

2021-05-25

Last modified:

2022-02-15

Imageset size:

2.15 TB

Imageset DOI:

10.6019/EMPIAR-10596

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi frame micographs of CARD8-DPP9-VbP-noTILT

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1811

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.82, 0.82)

Details:

For CARD8-DPP9-VbP 1,811 movies were collected at varying defocus levels ranging between −1.1 to −3.4 μm. One shot was recorded for each of the four holes per stage movement through image shift. All movies were exposed with a total dose of 67.06 e-/Å2 for 1.5 s fractionated over 50 frames. "references" folder contains the references.

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2. Unaligned multi frame micographs of CARD8-DPP9-VbP-TILT

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6642

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.82, 0.82)

Details:

For CARD8-DPP9-VbP tilt data, 6,642 movies at a stage tilt of 37° were collected at varying defocus levels ranging between −1.1 to −3.4 μm. One shot was recorded for each of the four holes per stage movement through image shift. All movies were exposed with a total dose of 67.06 e-/Å2 for 1.5 s fractionated over 50 frames. "references" folder contains the references.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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