EMPIAR-10589 - cryo-FIB and cryo-ET study of ribosome and polysome structures in E. coli [3 tilt series in MRC format]

EMPIAR-10589

cryo-FIB and cryo-ET study of ribosome and polysome structures in E. coli [3 tilt series in MRC format]

Publication:

Solvent quality and chromosome folding in Escherichia coli

Xiang YJ, Chang YJ, Liu J, Jacobs-Wagner C

(---)

Related EMDB entry:

EMD-22877 EMD-22878

Deposited:

2020-10-17

Released:

2021-06-18

Last modified:

2021-06-18

Imageset size:

9.65 GB

Imageset DOI:

10.6019/EMPIAR-10589

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Unaligned tilt series of cryo-FIB milling E. coli cells

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(5.457, 5.457)

Details:

1. Imaging parameters: Titan Krios, K2, ~100 total electron dose per angstrom square, bi-directional from ~0 degree.
2. Ecoli_001.mrc: collect positive angle images first, then negative angle images.
3. Ecoli_002.mrc and Ecoli_003.mrc: collect negative angle images first, then positive angle images.
4. The corresponding .tlt files are the tilt angles after compensate the tilt between the lamella and the grid.

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Citations

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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