EMPIAR-10065 - Lambda excision HJ intermediate [stack of 66033 particles in MRC format]

EMPIAR-10065

Lambda excision HJ intermediate [stack of 66033 particles in MRC format]

Publication:

Structure of a Holliday junction complex reveals mechanisms governing a highly regulated DNA transaction

Laxmikanthan G, Xu C, Brilot AF, Warren D, Steele L, Seah N, Tong W, Grigorieff N, Landy A, Van Duyne GD

eLife 5 (2016)

PMID: 27223329

DOI: 10.7554/eLife.14313

Related EMDB entry:

EMD-3400

Deposited:

2016-06-18

Released:

2016-06-20

Last modified:

2016-06-20

Imageset size:

4.04 GB

Imageset DOI:

10.6019/EMPIAR-10065

Experimental metadata:

Download xml json

Contains:

picked particles - multiframe - processed

Image Sets

1. Particle stack and Frealign parameter file

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

66033

Image size:

(128, 128)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(2.8, 2.8)

Details:

Particle stack contains images normalized with zero average and constant variance. The particle density is dark on light background. The data were collected on a Falcon II detector mounted on an FEI F30 microscope running at 300 kV. The Frealign parameter file contains the final particle alignments and classification results for the best class that was used to calculate the published 11-Å reconstruction. The table at the end of the parameter file does not contain the final FSC curve; the final FSC curve was calculated using more tightly masked half reconstructions (see related publication).

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Citations

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Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

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Conserved hydrophilic checkpoints tune FocA-mediated formate:H<sup>+</sup> symport.

Tüting C, Janson K, Kammel M, Ihling C, Lorenz J, Kyrilis FL, Hamdi F, Erdmann C, Sinz A, Sawers RG, Kastritis PL. (2025)

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