EMPIAR-10065
Lambda excision HJ intermediate [stack of 66033 particles in MRC format]
Publication:

Structure of a Holliday junction complex reveals mechanisms governing a highly regulated DNA transaction

Laxmikanthan G, Xu C, Brilot AF, Warren D, Steele L, Seah N, Tong W, Grigorieff N, Landy A, Van Duyne GD

eLife 5 (2016)

PMID: 27223329

Related EMDB entry:
Deposited:
2016-06-18
Released:
2016-06-20
Last modified:
2016-06-20
Imageset size:
4.04 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • picked particles - multiframe - processed
1. Particle stack and Frealign parameter file
Category:
picked particles - multiframe - processed
Image format:
MRC
No. of images or tilt series:
66033
Image size:
(128, 128)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(2.8, 2.8)
Details:
Particle stack contains images normalized with zero average and constant variance. The particle density is dark on light background. The data were collected on a Falcon II detector mounted on an FEI F30 microscope running at 300 kV. The Frealign parameter file contains the final particle alignments and classification results for the best class that was used to calculate the published 11-Å reconstruction. The table at the end of the parameter file does not contain the final FSC curve; the final FSC curve was calculated using more tightly masked half reconstructions (see related publication).
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