EMPIAR-13642
Cryo-EM single particle micrographs of hypomethylated 80S ribosomes [multiple data sets in EER and TIFF formats]
Publication:

2'-O-Methylation maintains ribosome structural and translation integrity

Zhao Y, Rai J, Cohen LN, Shu Y, Xu C, Goswami H, Chen X, Jin H, Marchand V, Motorin Y, Ghalei H, Li H

Molecular cell 86 (2026) 1495-1510.e7

PMID: 41928510

Deposited:
2024-03-01
Released:
2026-06-25
Last modified:
2026-06-25
Imageset size:
24.15 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. unaligned multi-frame micrographs of yeast hypomethylated 80S ribosome treated with hygromycin B
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
9063
Image size:
(4096, 4096)
Pixel type:
BIT
Pixel spacing:
(0.365, 0.365)
Details:
This dataset was collected on Falcon 4 camera in EER mode using Leginon, with a super-resolution pixel size of 0.365 and a total dose of 55.20 e–/Å2. The authors used EER fractionation of 32 and dose per frame of 0.82 with a binning factor 2 for motion correction.
Files:
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2. unaligned multi-frame micrographs of yeast nutrient limiting hypomethylated 80S ribosome
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
10148
Image size:
(11520, 8184)
Pixel type:
BIT
Pixel spacing:
(0.53, 0.53)
Details:
This dataset was collected on K3 camera at 81k magnification using EPU, with a super-resolution pixel size of 0.53 and a total dose of 60 e–/Å2 spreading over 60 frames. Gain file is also provided. Gain orientation in Relion: no rotation, Flip upside-down; in cryosparc: no rotation, no flip.
Files:
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3. unaligned multi-frame micrographs of yeast hypomethylated polysomes at 81K magnification
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7659
Image size:
(11520, 8184)
Pixel type:
BIT
Pixel spacing:
(0.53, 0.53)
Details:
This dataset was collected on K3 camera at 81k magnification using EPU, with a super-resolution pixel size of 0.53 and a total dose of 60 e–/Å2 spreading over 60 frames. Gain file is also provided. Gain orientation in Relion: no rotation, Flip upside-down; in cryosparc: no rotation, no flip.
Files:
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4. unaligned multi-frame micrographs of yeast hypomethylated polysomes at 105K magnification
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5585
Image size:
(11520, 8184)
Pixel type:
BIT
Pixel spacing:
(0.415, 0.415)
Details:
This dataset was collected on K3 camera at 81k magnification using EPU, with a super-resolution pixel size of 0.415 and a total dose of 60 e–/Å2 spreading over 60 frames. Gain file is also provided. Gain orientation in Relion: no rotation, Flip upside-down; in cryosparc: no rotation, no flip.
Files:
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5. unaligned multi-frame micrographs of yeast hypomethylated 80S ribosome bound with TSV-IRES, eEF2, sodarin
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7369
Image size:
(11520, 8184)
Pixel type:
BIT
Pixel spacing:
(0.53, 0.53)
Details:
This dataset was collected on K3 camera at 81k magnification using serialEM, with a super-resolution pixel size of 0.53 and a total dose of 60 e–/Å2 spreading over 60 frames. Gain file is also provided.
Files:
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