EMPIAR-13387
Electron tomography reveals perturbed inter-organellar relationship in PbSFA mutants [multiple data sets in MRC format]
Publication:

Essential connection between nucleus and apical pole maintains fidelity during Plasmodium progeny formation across the vertebrate and mosquito hosts

He B

The EMBO Journal (---)

Related EMDB entry:
Deposited:
2026-03-19
Released:
2026-06-23
Last modified:
2026-06-23
Imageset size:
27.23 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • reconstructed volumes
1. Electron tomograms of WT budding sporozoites at initiation stage
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
1
Image size:
(None, 384)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.9058, 1.9058)
Details:
The tomogram used to generate figure8A was named Tomo_WT_Initiation, the tilt series was recorded at 11500X, resulted in a pixel size of 0.953 nm. The tomogram was binned by 2 for processing so the pixel size is 1.9058 nm.
Image size 2986 x 3068 pixels, 384 sections
The segmentation was named Segmentation_WT_initiation
Files:
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2. Electron tomograms of WT budding sporozoites at elongation stage
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
1
Image size:
(None, 645)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.9058, 1.9058)
Details:
The tomogram used to generate figure8B was named Tomo_WT_elongation, the tilt series was recorded at 11500X, resulted in a pixel size of 0.953 nm. The tomogram was binned by 2 for processing so the pixel size is 1.9058 nm.
Image size 3076 x 3452, 645 sections
The segmentation was named Segmentation_WT_elongation
Files:
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3. Electron tomograms of SFA1KO budding sporozoites at initiation stage
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
1
Image size:
(None, 357)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.9058, 1.9058)
Details:
The tomogram used to generate figure8C was named Tomo_SFA1KO_initiation, the tilt series was recorded at 11500X, resulted in a pixel size of 0.953 nm. The tomogram was binned by 2 for processing so the pixel size is 1.9058 nm.
Image size 2687 x 2330, 357 sections.
The segmentation was named Segmentation_SFA1KO_initiation
Files:
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4. Electron tomograms of SFA1KO budding sporozoites at elongation stage
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
2
Image size:
(None, 899)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.9058, 1.9058)
Details:
The tomogram used to generate figure8D was named Tomo_SFA1KO_elongation, the tilt series was recorded at 11500X, resulted in a pixel size of 0.953 nm. The tomogram was binned by 2 for processing so the pixel size is 1.9058 nm.
Image size 2826 x 2811, 899 sections.
The segmentation was named Segmentation_SFA1KO_elongation, and was drawn on the further binned tomogram Tomo_SFA1KO_elongation_bin with pixel size of 3.8116 nm
Files:
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5. Electron tomograms of SFA2KO budding sporozoites at initiation stage
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
2
Image size:
(None, 342)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.9058, 1.9058)
Details:
The tomogram used to generate figure8E was named Tomo_SFA2KO_initiation, the tilt series was recorded at 11500X, resulted in a pixel size of 0.953 nm. The tomogram was binned by 2 for processing so the pixel size is 1.9058 nm.
Image size 2257 x 2251, 342 sections.
The segmentation was named Segmentation_SFA2KO_initiation, and was drawn on a older version of tomogram Tomo_SFA2KO_initiation_old, the quality of which was not ideal. This one was uploaded only to help visualize the segmentation
Files:
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6. Electron tomograms of SFA2KO budding sporozoites at elongation stage
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
1
Image size:
(None, 780)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(2.236, 2.236)
Details:
The tomogram used to generate figure8F was named Tomo_SFA2KO_elongation, the tilt series was recorded at 9600X, resulted in a pixel size of 1.12 nm. The tomogram was binned by 2 for processing so the pixel size is 2.236 nm.
Image size 2674 x 2679, 780 sections.
The segmentation was named Segmentation_SFA2KO_elongation
Files:
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Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)
Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)
He L, Bartesaghi A. (2026)
Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)
Kong L, Zottig X, Elferich J, Grigorieff N. (2026)
Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)
Gyawali R, Dhakal A, Wang L, Cheng J. (2026)
Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)
Jones HN, Deshmukh A, Pande K. (2026)
Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)