EMPIAR-13274
Single-particle Cryo Electron Microscopy of Human Cytomegalovirus Protease Bound to an Inhibitory Antibody Fragment [multiple data sets in EER and MRC formats]
Publication:

Antibody Reveals Conformational Latch Controlling Herpesvirus Proteases

Zimanyi M, Hulce KR, Bohn M, Norman J, Rohweder PJ, Cheng Y, Craik CS, Detomasi TC

bioRxiv (---)

Related EMDB entry:
Deposited:
2026-01-28
Released:
2026-07-12
Last modified:
2026-07-12
Imageset size:
7.39 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - single frame - processed
1. Unaligned multi-frame micrographs of human cytomegalovirus protease in complex with an inhibitory antibody fragment
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
16378
Image size:
(None, None)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.743, 0.743)
Details:
There are movies from 2 different grids in this dataset that were collected in a single krios session. In MZJ_1, the grids are Quantifoil 1.2/1.3 400 mesh copper foil grids, and MZJ_3 are UltrAUfoil 1.2/1.3 300 mesh grids. The MZJ_3 dataset tends to yield better particles, and our final reconstructions were comprised of about 80 percent particles from MZJ_3.
Files:
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2. Unclassified particles of human cytomegalovirus protease bound to an inhibitory antibody fragment
Category:
picked particles - single frame - processed
Image format:
MRC
No. of images or tilt series:
1455383
Image size:
(None, None)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(None, None)
Details:
All particles used in final reconstructions. Upon classification, at least 3 poses of the protease relative to the Fab can be resolved.
Files:
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