EMPIAR-12995 - Cryo-EM structure of R-eLACCO2 in the lactate-bound state [8250 multi-frame micrographs composed of 48 frames each in TIFF format]

EMPIAR-12995

Cryo-EM structure of R-eLACCO2 in the lactate-bound state [8250 multi-frame micrographs composed of 48 frames each in TIFF format]

Publication:

A red fluorescent genetically encoded biosensor for in vivo imaging of extracellular L-lactate dynamics

Kamijo Y, Mächler P, Ness N, Vu CQ, Kusakizako T, Mannuthodikayil J, Ku Z, Boisvert M, Grebenik E, Miyazaki I, Hashizume R, Sato H, Liu R, Hori Y, Tomita T, Katayama T, Furube A, Caraveo G, Paquet M-E, Drobizhev M, Nureki O, Arai S, Brancaccio M, Campbell RE, Kleinfeld D, Nasu Y

Nature Communications 16 (2025)

PMID: N/A

DOI: 10.1038/s41467-025-64484-x

Related EMDB entry:

EMD-60263

Deposited:

2025-09-19

Released:

2025-10-30

Last modified:

2025-10-30

Imageset size:

1.71 TB

Imageset DOI:

10.6019/EMPIAR-12995

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of R-eLACCO2 in the lactate-bound state

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8250

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 50 e/A2 over 48 frames

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Citations

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He L, Bartesaghi A. (2026)

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Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

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Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

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Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

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Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)