EMPIAR-12808 - Cryo Electron Microscopy of hIAPP and rIAPP fibrils in different aggregation solutions [stack of 1200 particles in MRCS format]

EMPIAR-12808

Cryo Electron Microscopy of hIAPP and rIAPP fibrils in different aggregation solutions [stack of 1200 particles in MRCS format]

Publication:

Cryo-Electron Microscopy Provides Mechanistic Insights into Solution-Dependent Polymorphism and Cross-Aggregation Phenomena of the Human and Rat Islet Amyloid Polypeptides

Valli DV, Maj MM

Biochemistry (2025)

DOI: 10.1021/acs.biochem.5c00042

Related EMDB entry:

Deposited:

2025-05-15

Released:

2025-11-21

Last modified:

2025-11-21

Imageset size:

737.25 GB

Imageset DOI:

10.6019/EMPIAR-12808

Experimental metadata:

Download xml json

Contains:

picked particles - multiframe - processed

Image Sets

1. Extracted particle stacks of co-aggregates formed by human and rat IAPP (hIAPP and rIAPP) at varying ratios and in different buffer conditions.

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

1200

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

This dataset contains a randomized subset of RELION-extracted particle stacks of amyloid fibrils formed by co-aggregation of human and rat islet amyloid polypeptide (hIAPP and rIAPP) under various conditions. The dataset is organized into four top-level directories based on the aggregation buffer used, and each is further subdivided into four directories corresponding to different hIAPP:rIAPP ratios (1:0, 1:1, 1:4, and 1:20).

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Citations

GTpick: A deep neural network for Cryo-EM particle detection.

Ni S, Yang C, Liu Y, Zhang Y, Shi Y, Qian A, Kong R, Chang S. (2025)

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Localization of macromolecules in crowded cellular cryo-electron tomograms from extremely sparse labels.

Uddin MR, Ahmed AY, Tabib HMS, Tahmid MT, Alam MZU, Freyberg Z, Xu M. (2025)

Structural insight into bicarbonate-mediated carboxylation by human vitamin K-dependent carboxylase.

Wu K, Wang Z, Yao D, Li S, Wang X, Zhang Y, Cao M, Shen Y, Xing S, Wu J, Lei M, Lan P. (2025)

Conserved hydrophilic checkpoints tune FocA-mediated formate:H<sup>+</sup> symport.

Tüting C, Janson K, Kammel M, Ihling C, Lorenz J, Kyrilis FL, Hamdi F, Erdmann C, Sinz A, Sawers RG, Kastritis PL. (2025)

Icecream: High-Fidelity Equivariant Cryo-Electron Tomography

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2025)

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