EMPIAR-12601
Cryo-EM structure of S.cerevisiae CHD1[L886G/L889G/L891G]-80N0/+1bp nucleosome complex [multiple data sets in TIFF and MRCS formats]
Publication:

A Competitive Regulatory Mechanism of the Chd1 Remodeler is Integral to Distorting Nucleosomal DNA

Nodelman IM, Folkwein HJ, Glime WS, Glime WS, Armache JP

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Deposited:
2024-08-13
Released:
2026-05-31
Last modified:
2026-05-31
Imageset size:
8.45 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - single frame - processed
1. Unaligned movies
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
17624
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.5252, 0.5252)
Details:
The images were collected as movies in Super-Resolution mode at nominal magnification of 81,000x with a physical pixel size of 1.0504 Å (super resolution of 0.5252 Å), resulting in 17,624 images, each fractionated into 40 frames accumulating 50 e- per Å2 (0.0825 s per frame, 1.25 e- per Å per frame) total dose.
Files:
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2. Optimized particles polished using "Bayesian Polishing" in Relion
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
17428
Image size:
(320, 320)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.0504, 1.0504)
Details:
Particle positions in star files. Stacks in mrcs files.
Files:
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