EMPIAR-12146 - Cryo-EM structure of uropathogenic Escherichia coli CysK:CdiA:tRNA complex [7044 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12146

Cryo-EM structure of uropathogenic Escherichia coli CysK:CdiA:tRNA complex [7044 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Mechanism of activation of contact-dependent growth inhibition tRNase toxin by the amino acid biogenesis factor CysK in the bacterial competition system

Feng Z, Yashiro Y, Tomita K

Nucleic acids research (2024)

PMID: 39228374

DOI: 10.1093/nar/gkae735

Related EMDB entry:

EMD-60564 EMD-60561 EMD-60563 EMD-60562

Deposited:

2024-06-20

Released:

2024-09-26

Last modified:

2024-09-26

Imageset size:

1.54 TB

Imageset DOI:

10.6019/EMPIAR-12146

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Imageset

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7044

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Microscope: FEI TITAN Krios
- Acceleration voltage (kV): 300
- Pixel size (Å/pix): 0.83
- Spherical aberration (Cs) : 2.7
- Nominal Defocus (nm): -800 to -1800
- Detector: Gatan K3 BIOQUANTUM
- Average electron dose per image (e-/A2): 50
- Number of frames per image: 50
- Please use gain.mrc as a gain reference.
- In CryoSPARC, select "flip gain ref in Y".

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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