EMPIAR-11840 - Structure of BARD1 ARD-BRCTs in complex with H2AKc15ub nucleosomes [multiple data sets in EER and MRC formats]

EMPIAR-11840

Structure of BARD1 ARD-BRCTs in complex with H2AKc15ub nucleosomes [multiple data sets in EER and MRC formats]

Publication:

BRCA1-BARD1 combines multiple chromatin recognition modules to bridge nascent nucleosomes

Burdett H, Foglizzo M, Musgrove LJ, Kumar D, Clifford G, Campbell LJ, Heath GR, Zeqiraj E, Wilson MD

Nucleic acids research 51 (2023) 11080-11103

PMID: 37823591

DOI: 10.1093/nar/gkad793

Related EMDB entry:

EMD-17928 EMD-16859

Deposited:

2023-08-25

Released:

2024-02-01

Last modified:

2024-02-01

Imageset size:

10.44 TB

Imageset DOI:

10.6019/EMPIAR-11840

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned multiframe movies in the EER format of BARD1 ARD-BRCTs in complex with H2AKc15ub nucleosomes

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

16009

Image size:

(None, None)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.86, 0.86)

Details:

A dose per physical pixel/s of 5.38 was used, resulting in a total electron dose of 36.4 e-/Å2, fractionated across 172 EPU frames. These were then grouped into 26 frames, resulting in an electron dose of 0.8 e-/Å2 per frame.

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2. Aligned micrographs of BARD1 ARD-BRCTs in complex with H2AKc15ub nucleosomes

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

16009

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.86, 0.86)

Details:

Raw movie frames are aligned with RELION's own implementation of the MotionCor2 algorithm using an EER fractionation of 45.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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