EMPIAR-11708 - Cryo-EM structure of the histamine-bound histamine H4 receptor and Gq complex [multiple data sets in TIFF format]

EMPIAR-11708

Cryo-EM structure of the histamine-bound histamine H4 receptor and Gq complex [multiple data sets in TIFF format]

Publication:

Structural insights into the agonists binding and receptor selectivity of human histamine H₄ receptor

Im D, Kishikawa JI, Shiimura Y, Hisano H, Ito A, Fujita-Fujiharu Y, Sugita Y, Noda T, Kato T, Asada H, Iwata S

Nature communications 14 (2023)

PMID: 37863901

DOI: 10.1038/s41467-023-42260-z

Related EMDB entry:

EMD-33785 EMD-33786

Deposited:

2023-09-29

Released:

2024-08-23

Last modified:

2025-08-22

Imageset size:

3.09 TB

Imageset DOI:

10.6019/EMPIAR-11708

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Cryo-EM structure of the histamine-bound histamine H4 receptor and Gq complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6300

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.88, 0.88)

Details:

- Please use 'CountCDSRef_20220210_IwataLab_96_0000_Feb10_14.54.40.mrc' as a gain reference without rotation or flipping in RELION (Y flipping is necessary for CryoSPARC).
- Movies were recorded on a Titan Krios with a Cs-corrector.
- The residual Cs value reported by the Cs corrector is 0.06216 mm.
- Four digits (0000 to 0017) in the file names represent image shift directions but grouping was not applied during processing.
- Flux at sample: ~ 1 e/A2/frame

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2. Cryo-EM structure of the imetit-bound histamine H4 receptor and Gq complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6462

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.88, 0.88)

Details:

- Please use 'CountCDSRef_20211202_IwataLab_99_0000_Dec02_14.17.09.mrc' as a gain reference without rotation or flipping in RELION (Y flipping is necessary for CryoSPARC).
- Movies were recorded on a Titan Krios with a Cs-corrector.
- The residual Cs value reported by the Cs corrector is 0.04223 mm.
- Four digits (0000 to 0017) in the file names represent image shift directions but grouping was not applied during processing.
- Flux at sample: ~ 1 e/A2/frame

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

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Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

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Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

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CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)