EMPIAR-11683
3D reconstructions of parasite development and the intracellular niche of the microsporidian pathogen E. intestinalis [multiple data sets in DM4 format]
Publication:

3D reconstructions of parasite development and the intracellular niche of the microsporidian pathogenE. intestinalis

Riggi MR, Antao NV, Lam C, Davydov A, Sall J, Petzold C, Liang F, Iwasa J, Ekiert DC, Bhabha G

bioRxiv (2023)

PMID: 37425741

Related EMDB entry:
Deposited:
2023-09-06
Released:
2023-10-17
Last modified:
2023-10-17
Imageset size:
537.94 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
1. 24h infected cell 1
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
86
Image size:
(20000, 35000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(20.0, 20.0)
Details:
Unaligned and unprocessed micrograph stack of a Vero cell at 24 hours post infection with E. intestinalis. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
Files:
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2. 24h infected cell 2
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
116
Image size:
(30000, 25000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(20.0, 20.0)
Details:
Unaligned and unprocessed micrograph stack of a Vero cell at 24 hours post infection with E. intestinalis. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
Files:
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3. 48h infected cell 1
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
200
Image size:
(12000, 12000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(20.0, 20.0)
Details:
Unaligned and unprocessed micrograph stack of a Vero cell at 48 hours post infection with E. intestinalis. Slice thickness=30nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
Files:
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4. 48h infected cell 2
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
83
Image size:
(22000, 23000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(20.0, 20.0)
Details:
Unaligned and unprocessed micrograph stack of a Vero cell at 48 hours post infection with E. intestinalis. There are two ROIs associated with this infected cell: ROI00 (22000x23000) and ROI01 (22000x23000). ROI00 and ROI01 were stitched together for segmentation analysis. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
Files:
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5. 48h infected cell 3
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
79
Image size:
(25000, 20000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(20.0, 20.0)
Details:
Unaligned and unprocessed micrograph stack of a parasitophorous vacuole in a Vero cell at 48 hours post infection with E. intestinalis. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
Files:
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6. 48h infected cell 4
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
74
Image size:
(14000, 15000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(20.0, 20.0)
Details:
Unaligned and unprocessed micrograph stack of a parasitophorous vacuole in a Vero cell at 48 hours post infection with E. intestinalis. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
Files:
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7. 48h infected cell 5
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
50
Image size:
(14000, 15000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(20.0, 20.0)
Details:
Unaligned and unprocessed micrograph stack of a parasitophorous vacuole in a Vero cell at 48 hours post infection with E. intestinalis. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
Files:
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8. Purified spores dataset 1
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
140
Image size:
(5000, 5000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(20.0, 20.0)
Details:
E. intestinalis spores purified from Vero cells. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
Files:
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9. Purified spores dataset 2
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
140
Image size:
(5000, 5000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(20.0, 20.0)
Details:
E. intestinalis spores purified from Vero cells. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
Files:
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10. Uninfected cell 1
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
50
Image size:
(12500, 12500)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(25.0, 25.0)
Details:
Partial volume of an uninfected Vero cell. Slice thickness=60nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
Files:
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11. Uninfected cell 2
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
50
Image size:
(12500, 12500)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(25.0, 25.0)
Details:
Partial volume of an uninfected Vero cell. Slice thickness=60nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
Files:
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Files:
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