EMPIAR-11450
Cryo electron tomography of Cytochalasin D-induced protrusions of Drosophila S2 cells - Datasets 1 - 4 [multiple data sets in TIFF and MRC formats]
Publication:

CryoET shows cofilactin filaments inside the microtubule lumen

Ventura Santos C, Rogers SL, Carter AP

bioRxiv (2023)

Related EMDB entry:
Deposited:
2023-02-10
Released:
2023-07-10
Last modified:
2023-07-10
Imageset size:
445.99 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • reconstructed volumes
1. Dataset 1: Raw multi-frame tilt images of Cytochalasin D induced protrusions of Drosophila S2 cells
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
25
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.952, 2.952)
Details:
Data from Dataset 1. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours. The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc folder.
Files:
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2. Dataset 1: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
25
Image size:
(1124, 1124)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(11.808, 11.808)
Details:
Dataset 1: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP.
Files:
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3. Dataset 2: Raw multi-frame tilt images of Cytochalasin D induced protrusions of Drosophila S2 cells
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
17
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.952, 2.952)
Details:
Data from Dataset 2. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours. The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc folder.
Files:
Loading...
4. Dataset 2: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
17
Image size:
(960, 928)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(11.808, 11.808)
Details:
Dataset 2: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP.
Files:
Loading...
5. Dataset 3: Raw multi-frame tilt images of Cytochalasin D induced protrusions of Drosophila S2 cells
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
39
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.952, 2.952)
Details:
Data from Dataset 3. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours. The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc folder.
Files:
Loading...
6. Dataset 3: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
39
Image size:
(960, 928)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(11.808, 11.808)
Details:
Dataset 3: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP.
Files:
Loading...
7. Dataset 4: Raw multi-frame tilt images of Cytochalasin D induced protrusions of Drosophila S2 cells
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
22
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.952, 2.952)
Details:
Data from Dataset 4. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 5 uM Cytochalasin D for 4 hours. The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc folder.
Files:
Loading...
8. Dataset 4: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
22
Image size:
(960, 928)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(11.808, 11.808)
Details:
Dataset 4: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP.
Files:
Loading...
Files:
Loading...

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