EMPIAR-11372 - Ubiquitinated FANCI in complex with FANCD2 and double-stranded DNA [multiple data sets in TIFF format]

EMPIAR-11372

Ubiquitinated FANCI in complex with FANCD2 and double-stranded DNA [multiple data sets in TIFF format]

Publication:

Structural and biochemical basis of interdependent FANCI-FANCD2 ubiquitination

Lemonidis K, Rennie ML, Arkinson C, Chaugule VK, Clarke M, Streetley J, Walden H

The EMBO journal (2022)

PMID: 36385258

DOI: 10.15252/embj.2022111898

Related EMDB entry:

EMD-14694

Deposited:

2023-01-09

Released:

2023-01-25

Last modified:

2023-01-25

Imageset size:

2.89 TB

Imageset DOI:

10.6019/EMPIAR-11372

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of ubiquitinated FANCI in complex with FANCD2 and double-stranded DNA

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5414

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.023, 1.023)

Details:

Imaging was performed on JEOL CRYO ARM 300 microscope, equipped with a DE64 detector (Magnification: 120,000x, acceleration voltage: 300 kV, spherical aberration: 2.7 mm, electron exposure: 45.2 e/Å^2). Movies were recorded in MRC format and were subsequently LZW compressed in TIFF format using RELION. For CryoSPARC processing, the corresponding gain reference file needs to be flipped in both Y and X axes.

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2. Unaligned multi-frame movies of ubiquitinated FANCI in complex with FANCD2 and double-stranded DNA (energy filtered imaging)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5814

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.023, 1.023)

Details:

Imaging was performed on JEOL CRYO ARM 300 microscope, equipped with a DE64 detector and an in-column omega filter (Magnification: 120,000x, acceleration voltage: 300 kV, spherical aberration: 2.7 mm, electron exposure: 46.8 e/Å^2, slit width: 30 eV). Movies were recorded in MRC format and were subsequently LZW compressed in TIFF format using RELION. For CryoSPARC processing, the corresponding gain reference file needs to be flipped in Y axis.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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