EMPIAR-11313 - Principles of mitoribosomal small subunit assembly in eukaryotes [multiple data sets in TIFF format]

EMPIAR-11313

Principles of mitoribosomal small subunit assembly in eukaryotes [multiple data sets in TIFF format]

Publication:

Principles of mitoribosomal small subunit assembly in eukaryotes

Harper NJ, Burnside C, Klinge S

Nature 614 (2022) 175-181

PMID: 36482135

DOI: 10.1038/s41586-022-05621-0

Related EMDB entry:

EMD-27249 EMD-27250 EMD-26969 EMD-26970 EMD-26968 EMD-26966 EMD-26971 EMD-27251 EMD-26967

Deposited:

2022-10-21

Released:

2023-02-17

Last modified:

2023-02-17

Imageset size:

73.61 TB

Imageset DOI:

10.6019/EMPIAR-11313

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of Ccm1-bound yeast mtSSU assembly intermediates

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

31995

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.54, 0.54)

Details:

Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect 31,995 micrographs with a defocus range of -1 to -2.5 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 40 frames using a total dose of 36 e-/pixel/second (specimen pixel size of 1.08 Å/px) with an exposure time of 2 seconds and a total dose of 61.73 e-/Å^2. Gain correction was applied on the fly during collection.

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2. Unaligned multi-frame micrographs of Rsm22-bound yeast mtSSU assembly intermediates

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

14111

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.54, 0.54)

Details:

Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect 14,111 micrographs with a defocus range of -1 to -2.5 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 40 frames using a total dose of 36 e-/pixel/second (specimen pixel size of 1.08 Å/px) with an exposure time of 2 seconds and a total dose of 61.73 e-/Å^2. Gain correction was applied on the fly during collection.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Unaligned multi-frame micrographs of METTL17-bound human mtSSU assembly intermediates (dataset 1)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

37112

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.54, 0.54)

Details:

Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect a dataset totaling 37,112 micrographs with a defocus range of -0.7 to -1.8 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 42 frames using a total dose of 28.5 e-/pixel/second with an exposure time of 2.1 seconds, resulting in a total dose of 51 e-/Å^2. Gain correction was applied on the fly during collection.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Unaligned multi-frame micrographs of METTL17-bound human mtSSU assembly intermediates (dataset 2)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9990

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.54, 0.54)

Details:

Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect a dataset totaling 9,990 micrographs with a defocus range of -0.7 to -1.8 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 42 frames using a total dose of 28.5 e-/pixel/second with an exposure time of 2.1 seconds, resulting in a total dose of 51 e-/Å^2. Gain correction was applied on the fly during collection.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Files:

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Download a list of selected files.

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