EMPIAR-11299 - Reconstituted USP1, UAF1, FANCI, mono-ubiquitinated FANCD2 and the inhibitor ML323 [10998 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11299

Reconstituted USP1, UAF1, FANCI, mono-ubiquitinated FANCD2 and the inhibitor ML323 [10998 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cryo-EM reveals a mechanism of USP1 inhibition through a cryptic binding site

Rennie ML, Arkinson C, Chaugule VK, Walden H

Science advances 8 (2022) eabq6353-N/A

PMID: 36170365

DOI: 10.1126/sciadv.abq6353

Related EMDB entry:

EMD-14720 EMD-15284 EMD-14722 EMD-14721 EMD-14719

Deposited:

2022-11-04

Released:

2022-11-15

Last modified:

2022-11-15

Imageset size:

2.32 TB

Imageset DOI:

10.6019/EMPIAR-11299

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of reconstituted USP1, UAF1, FANCI, monoubiquitinated FANCD2, DNA, and ML323

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10998

Image size:

(4092, 5760)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.06, 1.06)

Details:

Each multi-frame raw micrograph is composed of 40 frames each in compressed TIFF format, along with a separate gain reference. Multi-frame micrographs were collected at 81,000x nominal magnification at 15.46 e-/pix/s in super-resolution mode with 2x binning on a Gatan K3. Data collected on KriosIV at eBIC on 2021-11-29.
Sample contained 9.3 μM USP1, 9.3 μM UAF1, 1.8 μM FANCI, 1.8 μM FANCD2 monoubiquitinated on K561, 2.2 μM dsDNA (61 base pairs), and 18.3 μM ML323.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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