EMPIAR-11229
Single particle cryo-EM of KdpFAB(Ser162-P, D307N)C in apo condition [multiple data sets in TIFF format]
Publication:

Inhibited KdpFABC transitions into an E1 off-cycle state

Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hänelt I, Paulino C

eLife 11 (2022)

PMID: 36255052

Related EMDB entry:
Deposited:
2022-09-16
Released:
2022-11-16
Last modified:
2022-11-16
Imageset size:
2.49 TB
Imageset DOI:
Experimental metadata:
Contains:
  • micrographs - multiframe
1. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 1)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
787
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 51.5 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
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2. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 2)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1370
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 51.5 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
3. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 3)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2213
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 51.5 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
4. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 4)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1333
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 51.5 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
5. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 5)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
317
Image size:
(3838, 3838)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
6. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 6)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
618
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
7. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 7)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
181
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
8. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 8)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
448
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
9. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 9)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
93
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
10. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 10)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
959
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
11. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 11)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
862
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
12. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 12)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
897
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
13. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 13)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1248
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
14. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 14)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
584
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
15. Unaligned movie frames of the KdpFAB(Ser162-P, D307N)C without nucleotide in DDM (Dataset 15)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
954
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.012, 1.012)
Details:
Unaligned multi-frame movies with each 60 frames per movie collected at 200 kV in counting mode. Total accumulated electron exposure at specimen level corresponds to 53.3 electrons per Å^2. Movie are not gain corrected and the gain reference is provided.
Files:
Loading...
Files:
Loading...
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Citations
Liu T, Shilliday F, Cook AD, Zeeshan M, Brady D, Tewari R, Sutherland CJ, Roberts AJ, Moores CA. (2022)
Jespersen N, Ehrenbolger K, Winiger RR, Svedberg D, Vossbrinck CR, Barandun J. (2022)
Sheng Y, Harrison PJ, Vogirala V, Yang Z, Strain-Damerell C, Frosio T, Himes BA, Siebert CA, Zhang P, Clare DK. (2022)
See all citations
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