EMPIAR-11058
In situ cryo-electron tomography of T. kivui cells [multiple data sets in MRC and TIFF formats]
Publication:

Membrane-anchored HDCR nanowires drive hydrogen-powered CO2 fixation

Dietrich HM, Righetto RD, Kumar A, Wietrzynski W, Trischler R, Schuller SK, Wagner J, Schwarz FM, Engel BD, Müller V, Schuller JM

Nature 607 (2022) 823-830

PMID: 35859174

Related EMDB entry:
Deposited:
2022-05-29
Released:
2022-07-12
Last modified:
2022-07-29
Imageset size:
373.37 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • subtomograms
  • reconstructed volumes
  • tilt series
  • micrographs - multiframe
1. Subtomograms of HDCR filament particles at bin2
Category:
subtomograms
Image format:
MRC
No. of images or tilt series:
6727
Image size:
(96, 96)
Pixel type:
SIGNED BYTE
Pixel spacing:
(7.04, 7.04)
Details:
Particle coordinates and final orientations are given in file hdcr_tm_bin2_motl_12.star in STOPGAP format.
Files:
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2. Subtomograms of T. kivui ribosome particles at bin1
Category:
subtomograms
Image format:
MRC
No. of images or tilt series:
25141
Image size:
(180, 180)
Pixel type:
SIGNED BYTE
Pixel spacing:
(3.52, 3.52)
Details:
Particle coordinates and final orientations are given in file ribo_tm_bin1_motl_12.star in STOPGAP format.
Files:
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3. Denoised tomograms of T. kivui FIB-milled cells at bin4
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
20
Image size:
(928, 928)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(14.08, 14.08)
Details:
Tomograms were denoised with CRYO-CARE using reconstructions from odd and even frames.
Files:
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4. Tilt series of T. kivui FIB-milled cells - session 2020-10-22
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
19
Image size:
(3712, 3712)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(3.52, 3.52)
Details:
Tilt series as MRC stacks containing motion-corrected and averaged frames for each tilt. Associated .mdoc files contain the metadata associated with data collection. Corresponding imod/ directory contains IMOD metadata for reconstruction.
Files:
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5. Tilt series of T. kivui FIB-milled cells - session 2021-02-12
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
17
Image size:
(3712, 3712)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(3.52, 3.52)
Details:
Tilt series as MRC stacks containing motion-corrected and averaged frames for each tilt. Associated .mdoc files contain the metadata associated with data collection. Corresponding imod/ directory contains IMOD metadata for reconstruction.
Files:
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6. Unaligned raw frames of T. kivui FIB-milled cells tilt series - session 2020-10-22
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1030
Image size:
(3838, 3710)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(3.52, 3.52)
Details:
File names follow the pattern ___.tif
Gain reference (DM4): CountRef_000_-10.0_Oct22_10.57.24.dm4
Gain reference (MRC): CountRef_000_-10.0_Oct22_10.57.24.mrc
Defects list: defects_000_-10.0_Oct22_10.57.24.txt
Files:
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7. Unaligned raw frames of T. kivui FIB-milled cells tilt series - session 2021-02-12
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1057
Image size:
(3838, 3710)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(3.52, 3.52)
Details:
File names follow the pattern ___.tif
Gain reference (DM4): CountRef_2021-01-12_10.54.03_tomo_01_000_-10.0.dm4
Gain reference (MRC): CountRef_2021-01-12_10.54.03_tomo_01_000_-10.0.mrc
Defects list: defects_tomo_001_026_36.0_Jan11_12.51.02.txt
Files:
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Files:
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