EMPIAR-10930
Anaplastic lymphoma kinase (ALK) extracellular fragment of ligand binding region 648-1025 in complex with AUG-alpha [multiple data sets in TIFF and MRC formats]
Publication:

Mechanism for the activation of the anaplastic lymphoma kinase receptor

Reshetnyak AV, Rossi P, Myasnikov AG, Sowaileh M, Mohanty J, Nourse A, Miller DJ, Lax I, Schlessinger J, Kalodimos CG

Nature 7887 (2021) 153-157

PMID: 34819673

Related EMDB entry:
Deposited:
2022-01-05
Released:
2022-01-28
Last modified:
2022-01-28
Imageset size:
10.23 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
1. Unaligned multi-frame micrographs of 2:2 ALK:AugAlpha complex
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
13618
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.413, 0.413)
Details:
Raw movie frames. Movies were recorded at defocus values from −0.8 to −1.8 m at 105,000× magnification, corresponding to a pixel size of 0.826 Å at the specimen level (super-resolution pixel size is 0.413). Seventy frames with 2.8-s exposure (0.04 s per frame and the dose of 1.2 e per frame per A2) were collected with the total dose of around 88.4 e, and the first frame was discarded.
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2. Aligned single-frame micrographs of 2:2 ALK:AugAlpha complex
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
13618
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.826, 0.826)
Details:
Aligned micrographs. Motion correction was performed on raw super-resolution movie stacks and binned by 2 using MotionCor2

data/Particles/ - Particles were automatically picked using cisTEM. Here we provide two star files, so that someone can extract particles in Relion using motion-corrected images and one of the star files (final structure and all picked particles) with the coordinates. Box size used for reconstruction was 256.
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