EMPIAR-10924
Apo C-terminal half of LRRK2 (I2020T) bound to microtubule [2379 multi-frame micrographs composed of 50 frames each in TIFF format]
Publication:

Structural basis for Parkinson's disease-linked LRRK2's binding to microtubules

Snead DM, Matyszewski M, Dickey AM, Lin YX, Leschziner AE, Reck-Peterson SL

Nature structural & molecular biology 29 (2022) 1196-1207

PMID: 36510024

Related EMDB entry:
Deposited:
2022-01-08
Released:
2023-01-03
Last modified:
2023-01-03
Imageset size:
643.89 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of apo microtubule bound C-terminus of Leucine Rich Repeat Kinase 2 (LRRK2) (I2020T)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2379
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.16, 1.16)
Details:
Contains data collected from 2 grids (19dec14f and 20aug12b). Each folder contains its own gain reference with the "_norm.mrc" suffix found in the references folder. Blank dark references are also included but are not needed for correction. Gain correction in cryosparc should work with default settings, while for motioncor2, please flip the image and apply no rotation.
Total dose is 55 e/A-2
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Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)