EMPIAR-10791
High resolution 3D imaging of liver subcellular architecture and its link to metabolic function [multiple data sets in TIFF format]
Publication:

Regulation of liver subcellular architecture controls metabolic homeostasis

Parlakgul G, Arruda AP, Pang S, Cagampan E, Min N, Guney E, Lee Y, Inouye K, Hess HF, Xu CS, Hotamisligil GS

Nature (2022)

PMID: 35264794

Related EMDB entry:
Deposited:
2021-08-13
Released:
2022-01-14
Last modified:
2022-03-11
Imageset size:
2.11 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • reconstructed volumes
1. 6461 Lean Liver Dataset - FIB-SEM and Segmentation
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
5638
Image size:
(12000, 8000)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(8.0, 8.0)
Details:
6461 is Lean liver dataset - 12000 x 8000 x 5638 voxel, 8nm isotropic resolution.
There are 5638 consecutive raw FIBSEM images
and 5638 corresponding images in each segmentation class
(ER, ER sheets, ER Tubules, Mitochondria, Lipid Droplet, Cell Membrane, Nuclear membrane)
Files:
Loading...
2. 6464 Obese1 Liver Dataset - FIB-SEM and Segmentation
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
7896
Image size:
(9112, 10200)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(8.0, 8.0)
Details:
6464 is Obese1 liver dataset, 9112 x 10200 x 7896 voxel, 8nm isotropic resolution
There are 7896 consecutive raw FIBSEM images
and 7896 corresponding images in each segmentation class
(ER, ER sheets, ER Tubules, Mitochondria, Lipid Droplet, Cell Membrane, Nuclear membrane)
Files:
Loading...
3. 9430 Obese2 Liver Dataset - FIB-SEM and Segmentation
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
8501
Image size:
(8000, 8050)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(8.0, 8.0)
Details:
9430 is Obese2 liver dataset, 8000 x 8050 x 8501 voxel, 8nm isotropic resolution
There are 8501 consecutive raw FIBSEM images
and 8501 corresponding images in each segmentation class
(ER, ER sheets, ER Tubules, Mitochondria, Lipid Droplet, Cell Membrane, Nuclear membrane)
Files:
Loading...
4. 1857 Obese Climp63 Liver Dataset - FIB-SEM and Segmentation
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
3629
Image size:
(9700, 9650)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(8.0, 8.0)
Details:
1857 is Obese Climp63 liver dataset, 9700 x 9650 x 3629 voxel, 8nm isotropic resolution
There are 3629 consecutive raw FIBSEM images
and 3629 corresponding images in each segmentation class
(ER, ER sheets, ER Tubules, Mitochondria, Lipid Droplet, Cell Membrane)
Files:
Loading...
5. Segmentation Evaluation files - Manual annotation, prediction, raw data files
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
25
Image size:
(150, 150)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(16.0, 16.0)
Details:
The evaluation was performed on down-sampled data. A down-sampled version of the raw data is provided as well. Raw data, manual annotation data and predictions are provided as tiff stacks (8bit or 32bit, compressed, packaged in a .zip container). For lipid droplets, mitochondria, nuclear membrane and ER, we selected 3 test volumes of 300×300×50 voxels at random locations. Object based scores were only calculated for mitochondria and lipid droplets. For ER sheet and tubule evaluation, we randomly choose an area of 750x750 pixel^2 at 4 different locations in each dataset. Raw data, manual annotations and predictions are provided.
Files:
Loading...
Files:
Loading...

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)