EMPIAR-10739
Single-particle cryo-EM dataset of the complex between ALC1 and a PARylated nucleosome [multiple data sets in TIFF and MRCS formats]
Publication:

Structure and dynamics of the chromatin remodeler ALC1 bound to a PARylated nucleosome.

Bacic L, Gaullier G, Sabantsev A, Lehmann LC, Brackmann K, Dimakou D, Halic M, Hewitt G, Boulton SJ, Deindl S

eLife 10 (2021)

PMID: 34486521

Related EMDB entry:
Deposited:
2021-06-21
Released:
2023-07-25
Last modified:
2023-07-25
Imageset size:
14.87 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - multiframe - processed
1. Unaligned movies of the complex between ALC1 and a PARylated nucleosome - batch 1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
14502
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.84, 0.84)
Details:
40 gain-corrected frames per movie, collected on Titan Krios with K3 detector in counting mode.
Files:
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2. Unaligned movies of the complex between ALC1 and a PARylated nucleosome - batch 2
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
19496
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.84, 0.84)
Details:
40 gain-corrected frames per movie, collected on Titan Krios with K3 detector in counting mode.
Files:
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3. Extracted particles at original pixel size - large set
Category:
picked particles - multiframe - processed
Image format:
MRCS
No. of images or tilt series:
426066
Image size:
(512, 512)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.84, 0.84)
Details:
Large set of particles, homogeneous in composition/stoichiometry but highly heterogeneous in conformation, as assessed by RELION Refine3D.
Files:
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4. Extracted particles at original pixel size - smaller subset
Category:
picked particles - multiframe - processed
Image format:
MRCS
No. of images or tilt series:
43698
Image size:
(512, 512)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.84, 0.84)
Details:
Smaller subset of particles, homogeneous in composition/stoichiometry but heterogeneous in conformation, as assessed by RELION Refine3D. Conformational heterogeneity was tractable using cryoSPARC 3DVA and cryoDRGN.
Files:
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Files:
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