EMPIAR-10712 - Transmission electron micrographs of mouse RNF213 incubated with ATPγS [multiple data sets in TIFF format]

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EMPIAR-10712

Transmission electron micrographs of mouse RNF213 incubated with ATPγS [multiple data sets in TIFF format]

Publication:

E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP

Ahel J, Clausen T, Virdee S, Fletcher AJ, Grabarczyk DB, Deszcz L, Lehner A, Roitinger E

BiorXiv (---)

Related EMDB entry:

EMD-12932

Deposited:

2020-06-17

Released:

2022-06-14

Last modified:

2022-06-14

Imageset size:

2.34 TB

Imageset DOI:

10.6019/EMPIAR-10712

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. unaligned multi-frame micrographs from a dataset collected without grid tilting

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5950

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.04, 1.04)

Details:

set1_noTilt/raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected
==
set1_noTilt/set1_GainReference.mrc == gain reference for the dataset
set1_noTilt/set1_micrographs_ctf.star == relion 3.1 star file describing the gain-corrected dose-weighed motion-corrected micrographs
set1_noTilt/micrographs_ctf_motioncorr/ == gain-corrected dose-weighed motion-corrected micrographs
==
set1_noTilt/coordinates_initial_cryolo/ == particle coordinates picked by cryolo
set1_noTilt/coordinates_final_refined/ == particle coordinates for the particles used in the final reconstruction
set1_noTilt/particles/ == particles used in the final reconstruction, Bayesian-polished by relion 3.1
==
./refined_particles_combined.star == relion 3.1 star file describing the particles after the final reconstruction, including angular assignment
==
./cryolo_YOLO_RNF213.h5 == cryolo network model used for initial particles picking
./cryolo_YOLO_RNF213_config.json == config file for the cryolo network model used for initial particles picking
./MTF_K2summit_300kV.star == MTF file used for MTF correction for this dataset

Files:

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2. unaligned multi-frame micrographs from a dataset collected with a 30 deg grid tilt

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3055

Image size:

(5760, 4092)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.052, 1.052)

Details:

set2_withTilt/raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected
==
set2_withTilt/set2_GainReference.mrc == gain reference for the dataset
set2_withTilt/set2_micrographs_ctf.star == relion 3.1 star file describing the gain-corrected dose-weighed motion-corrected micrographs
set2_withTilt/micrographs_ctf_motioncorr/ == gain-corrected dose-weighed motion-corrected micrographs
==
set2_withTilt/coordinates_initial_3Dpick/ == particle coordinates picked by the relion 3D template picker
set2_withTilt/coordinates_final_refined/ == particle coordinates for the particles used in the final reconstruction
set2_withTilt/particles/ == particles used in the final reconstruction, Bayesian-polished by relion 3.1
==
./refined_particles_combined.star == relion 3.1 star file describing the particles after the final reconstruction, including angular assignment
==
./mtf_k3_standard_300kV_FL2.star == MTF file used for MTF correction for this dataset

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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