EMPIAR-10595 - Human NLRP1-DPP9-VbP complex [multiple data sets in TIFF format]

EMPIAR-10595

Human NLRP1-DPP9-VbP complex [multiple data sets in TIFF format]

Publication:

DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation

Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H

Nature (2021)

PMID: 33731932

DOI: 10.1038/s41586-021-03350-4

Related EMDB entry:

EMD-22075

Deposited:

2020-12-15

Released:

2021-04-14

Last modified:

2021-04-14

Imageset size:

1.18 TB

Imageset DOI:

10.6019/EMPIAR-10595

Experimental metadata:

Download xml json

Contains:

micrographs - focal pairs - unprocessed
micrographs - multiframe

Image Sets

1. Unaligned multi frame micographs of NLRP1-DPP9-VbP-noTILT

Category:

micrographs - focal pairs - unprocessed

Image format:

TIFF

No. of images or tilt series:

3553

Image size:

(None, None)

Pixel type:

BIT

Pixel spacing:

(0.825, 0.825)

Details:

The defocus range between −0.8 to −2.2 μm and to record two shots for each of the four holes per stage movement through image shift. All movies were exposed with a total dose of 52.0 e-/Å2 for 2.0 s fractionated over 50 frames.

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2. Unaligned multi frame micographs of NLRP1-DPP9-VbP-TILT

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1954

Image size:

(None, None)

Pixel type:

BIT

Pixel spacing:

(0.825, 0.825)

Details:

An ideal tilt angle of 37° was calculated from this processed dataset using cryoEF. The defocus range between −1.5 to −3.0 μm with two shots recorded for each hole per stage movement through image shift. All movies were
exposed with a total dose of 65.0 e-/Å2 for 2.4 s fractionated over 43 frames

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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