EMPIAR-10252 - Catalytic subunit of protein kinase A bound to ATP, manganese, and IP20 movies obtained using Talos Arctica operating at 200 kV equipped with a K2 [4812 multi-frame micrographs composed of 44 frames each in MRC format]

EMPIAR-10252

Catalytic subunit of protein kinase A bound to ATP, manganese, and IP20 movies obtained using Talos Arctica operating at 200 kV equipped with a K2 [4812 multi-frame micrographs composed of 44 frames each in MRC format]

Publication:

High-resolution structure determination of sub-100 kilodalton complexes using conventional cryo-EM

Herzik Jr MA, Wu M, Lander GC

bioRxiv (2018)

DOI: 10.1101/489898

Related EMDB entry:

EMD-0409

Deposited:

2019-02-11

Released:

2019-03-25

Last modified:

2019-04-12

Imageset size:

619.55 GB

Imageset DOI:

10.6019/EMPIAR-10252

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (*/rawdata/*frames.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   5,884,597,772 kilobytes
Compressed size:   649,483,795 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Raw, unaligned movie stacks of the catalytic subunit of protein kinase A bound to ATP, manganese, and IP20 acquired on a Talos Arctica using a K2 direct electron detector

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4812

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.5585, 0.5585)

Details:

Raw, unaligned counting mode frame stacks (44 frames) of the catalytic subunit of protein kinase A bound to ATP, manganese, and IP20 in vitreous ice imaged on a Talos Arctica using a K2 direct electron detector. Three separate data collections – 18feb27c (collected without tilt), 18mar03b (collected without tilt), and 18aug21a (collected at a 30˚ tilt) – that were combined are provided. Images used for corrections are also provided (require flip in Y).

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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