EMPIAR-10171 - CryoET of apoferritin with 0.5 mM TCEP single particle with spot-to-plunge time of 170ms [multiple data sets in MRC and DYNAMO TBL AND OMD FILES formats]

EMPIAR-10171

CryoET of apoferritin with 0.5 mM TCEP single particle with spot-to-plunge time of 170ms [multiple data sets in MRC and DYNAMO TBL AND OMD FILES formats]

Publication:

Reducing effects of particle adsorption to the air-water interface in cryoEM

Noble AJ, Wei H, Dandey VP, Zhang Z, Potter CS, Carragher B

BioRxiv (---)

DOI: 10.1101/288340

Related EMDB entry:

EMD-7627

Deposited:

2018-04-03

Released:

2018-04-05

Last modified:

2018-04-05

Imageset size:

31.62 GB

Imageset DOI:

10.6019/EMPIAR-10171

Experimental metadata:

Download xml json

Contains:

tilt series
particle picking

Image Sets

1. Tilt-series image stacks

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3712, 4984)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(2.34, 2.34)

Details:

Not frame-aligned.
.mdoc files included.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

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Download a list of selected files.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Manual particle picking in Dynamo

Category:

particle picking

Image format:

DYNAMO TBL AND OMD FILES

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

Binned by 4 manual particle picking of the series0001 tomogram using Dynamo. Manual picking was performed to accurately distinguish between particles adsorbed to the air-water interfaces, non-adsorbed particles, and particles over or adsorbed to carbon.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Appion-Protomo tilt-series alignments

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3712, 4984)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(2.34, 2.34)

Details:

Includes a full tilt-series alignment run for each tilt-series. To view properly, navigate to the protomo_alignments folder in Appion-Protomo.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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