EMPIAR-10028 - Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine [multiple data sets in MRC format]

EMPIAR-10028

Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine [multiple data sets in MRC format]

Publication:

Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine

Wong W, Bai XC, Brown A, Fernandez IS, Hanssen E, Condron M, Tan YH, Baum J, Scheres SH

eLife 3 (2014)

PMID: 24913268

DOI: 10.7554/elife.03080

Related EMDB entry:

EMD-2660

Deposited:

2015-05-29

Released:

2015-06-17

Last modified:

2023-03-28

Imageset size:

1.17 TB

Imageset DOI:

10.6019/EMPIAR-10028

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - multiframe - processed

Image Sets

1. Unaligned multi-frame micrographs

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1081

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.34, 1.34)

Details:

Micrographs are split into two sub-directories: Micrographs_part1 and Micrographs_part2. Each of these directories contains BOTH the unaligned frames (xxx_movie.mrcs) and the frame-averaged micrographs (600.mrc). These directories also contain the ctffind3 log files and the autopick star files.

Files:

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2. Frame averaged micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

1081

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.34, 1.34)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Processed shiny particles

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

105247

Image size:

(360, 360)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.34, 1.34)

Details:

Particle images are organized in two sub-directories - MRC_0601 and MRC_1901. Particle images are in MRCS image stacks - one each corresponding to the micrograph from which they were picked. The text file shiny_2sets.star describes the picking of the particles from the micrographs.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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