• ftp directory of EMPIAR-10291_tutorial
• EMPIAR-10291_100mic.tar (5.4 GB)
• EMPIAR-10291_50mic.tar (2.7 GB)
• EMPIAR-10291_100mic_precalculated_results.tar (for Relion 3.1;14 GB)
• EMPIAR-10291_50mic_precalculated_results.tar (for Relion 3.1; 8.5 GB)
• Relion 3.1 tutorial document for preprocessing (in Japanese)
• Relion 3.1 tutorial for 2D_3D_classification (in Japanese)
• cryoSPARC 3.2.0 tutorial for INX-6_50mic
• cryoSPARC 3.2.0 results data for INX-6 50mic(cryoSPARC_INX-6_results_202106.zip;67 MB)
• Tutorial data for atomic modeling by Coot(EM_koushuukai.zip; 53 MB)

The original EMPIAR-10291 (15.9 GB) contains 300 mrc files, each of them has 3710 x 3838 x 1 (#MRC_MODE 2 "32-bit signed real').

1. Execute "CTFfind" by Relion 3.1 (CTFFIND-4.1).
2. Check CtfFind/*/micrographs_ctf.star

First, we checked a distribution of _rlnCtfFigureOfMerit #8 and _rlnCtfMaxResolution #9, and cofirmed it has a single-peak distribution. Then, we decided to choose good micrographs only by MaxResolution.

We have organized two workshops using this tutorial data

• CiCLE workshop (2021/01/18-19) using Relion 3.1
• CiCLE workshop (2021/06/24-25) using cryoSPARC 3.2.0

The images stored as EMPIAR-10248 collected on JEOL CRYO ARM 300, provides a very high resolution (1.54 angstrom) 3D density map for apo ferritin. However, this data is not good for educational workshops and exercises because analyzing this data set (145.9 Gbyte) requires large computational costs. We therefore provide a reduced data set (1.7 Gbyte) for an educational purpose.

• ftp directory of EMPIAR-10148_tutorial
• EMPIAR-10248_20movies.tar (1.7GB)
• EMPIAR-10248_20movies_precalculated_results.tar (12 GB)
• Tutorial document for preprocessing (in Japanese)
• Tutorial document for 2D_3D classification (in Japanese)

Procedure to reduce image data

1. For the 971 TIFF files in EMPIAR-10248, we executed MotionCorr and CTFfind using Realion 3.0.
2. Among the TIFF fiels of EMPIAR-10248 with _rlnCtfFigureOfMerit > 0.1, the 20 TIFF files with the best _rlnCtfMaxResolution have been taken.
3. Each TIFF file has 50 frames. We took only first 25 frames.