EMPIAR-13396
C. thermocellum UvrA in complex with DNA with an abasic site and AMPPNP [multiple data sets in TIFF and MRCS formats]
Publication:

Structural snapshots of the mechanism of ATP-dependent DNA damage recognition by UvrA

Nirwal S, Czarnocki-Cieciura M, Zajko W, Skowronek K, Szczepanowski RH, Nowotny M

Nature communications 17 (2025)

PMID: 41381534

Related EMDB entry:
Deposited:
2026-03-14
Released:
2026-03-27
Last modified:
2026-03-27
Imageset size:
2.75 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
  • picked particles - single frame - processed
1. Unaligned multi-frame movies of C. thermocellum UvrA in complex with DNA with an abasic site and AMPPNP (untilted movies)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
6678
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.84, 0.84)
Details:
Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU) with 0 deg stage tilt. Voltage: 300 kV; spherical aberration: 2.7; dose per frame: ~1 e/A^2 (total dose: 40.64 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.1, -1.8, -1.5, -1.2, -0.9 µm. Objective aperture retracted, C2 aperture: 50 µm. See microscope_settings.txt for details
Files:
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2. Unaligned multi-frame movies of C. thermocellum UvrA in complex with DNA with an abasic site and AMPPNP (tilted movies)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2994
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.84, 0.84)
Details:
Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU) with 30 deg stage tilt. Voltage: 300 kV; spherical aberration: 2.7; dose per frame: ~1 e/A^2 (total dose: 40.49 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.0, -1.5, -1.0 µm. Objective aperture retracted, C2 aperture: 50 µm. See microscope_settings.txt for details
Files:
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3. Aligned particles of C. thermocellum UvrA in complex with DNA with an abasic site and AMPPNP (ATP-bound conformation 1)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
197058
Image size:
(320, 320)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.84, 0.84)
Details:
Aligned particles with corresponding STAR file. Micrographs were initially binned 2x during Motion correction.
Files:
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4. Aligned particles of C. thermocellum UvrA in complex with DNA with an abasic site and AMPPNP (ATP-bound conformation 2)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
195233
Image size:
(320, 320)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.84, 0.84)
Details:
Aligned particles with corresponding STAR file. Micrographs were initially binned 2x during Motion correction.
Files:
Loading...