EMPIAR-12476 - single-particle cryoEM data of AsCas12f-sgRNA-DNA complex [7591 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12476

single-particle cryoEM data of AsCas12f-sgRNA-DNA complex [7591 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activity

Wu T, Liu C, Zou S, Lyu R, Yang B, Yan H, Zhao M, Tang W

Nature chemical biology 19 (2023) 1384-1393

PMID: 37400536

DOI: 10.1038/s41589-023-01380-9

Related EMDB entry:

EMD-27801

Deposited:

2024-12-03

Released:

2025-02-04

Last modified:

2025-02-04

Imageset size:

3.92 TB

Imageset DOI:

10.6019/EMPIAR-12476

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame data of AsCas12f-sgRNA-DNA complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7591

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.5325, 0.5325)

Details:

Total dose: 50 electrons per square angstrom. Gain image included as a mrc file.

Files:

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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