EMPIAR-12476 - single-particle cryoEM data of AsCas12f-sgRNA-DNA complex [7591 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12476

single-particle cryoEM data of AsCas12f-sgRNA-DNA complex [7591 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activity

Wu T, Liu C, Zou S, Lyu R, Yang B, Yan H, Zhao M, Tang W

Nature chemical biology 19 (2023) 1384-1393

PMID: 37400536

DOI: 10.1038/s41589-023-01380-9

Related EMDB entry:

EMD-27801

Deposited:

2024-12-03

Released:

2025-02-04

Last modified:

2025-02-04

Imageset size:

3.92 TB

Imageset DOI:

10.6019/EMPIAR-12476

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame data of AsCas12f-sgRNA-DNA complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7591

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.5325, 0.5325)

Details:

Total dose: 50 electrons per square angstrom. Gain image included as a mrc file.

Files:

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Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)