EMPIAR-12450 - Cryo-EM Structures of human LINE-1 ORF2p target-primed reverse transcription complex [multiple data sets in TIFF and MRCS formats]

EMPIAR-12450

Cryo-EM Structures of human LINE-1 ORF2p target-primed reverse transcription complex [multiple data sets in TIFF and MRCS formats]

Publication:

Structural mechanism of LINE-1 target-primed reverse transcription

Ghanim GE, Hu H, Boulanger J, Nguyen THD

Science (New York, N.Y.) (2025)

PMID: 40048554

DOI: 10.1126/science.ads8412

Related EMDB entry:

EMD-52072 EMD-52071 EMD-52073 EMD-52070

Deposited:

2024-08-20

Released:

2025-04-07

Last modified:

2025-04-07

Imageset size:

5.12 TB

Imageset DOI:

10.6019/EMPIAR-12450

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of the human LINE-1 ORF2p TPRT complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

25374

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.955, 0.955)

Details:

These are movie files in TIFF format. Associated gain reference for the dataset is also included in TIFF format.

Files:

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2. Polished particle stack of the ORF2p TPRT complex consensus reconstruction (EMD-52070)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

680273

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.955, 0.955)

Details:

---

Files:

This entry cannot be downloaded from PDBj.

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3. Polished particle stack of the ORF2p TPRT complex with the fingers domain in a closed conformation (EMD-52071)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

185228

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.955, 0.955)

Details:

---

Files:

This entry cannot be downloaded from PDBj.

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4. Polished particle stack of the ORF2p TPRT complex with the fingers domain in an open conformation (EMD-52072)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

183579

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.955, 0.955)

Details:

---

Files:

This entry cannot be downloaded from PDBj.

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5. Polished particle stack of the ORF2p TPRT complex with the EN domain resolved (EMD-52073)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

121941

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.955, 0.955)

Details:

---

Files:

This entry cannot be downloaded from PDBj.

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