EMPIAR-12294 - cryo electron tomography of Pickering-emulsified condensate (LAF based scaffolds and GFP(-9)-6his-mi3 cage protein) [400 reconstructed volumes in TIFF format]

EMPIAR-12294

cryo electron tomography of Pickering-emulsified condensate (LAF based scaffolds and GFP(-9)-6his-mi3 cage protein) [400 reconstructed volumes in TIFF format]

Publication:

Size-controlled synthesis of phase separated protein condensates with interfacial protein cages

Lee YS, Oh HJ, Hwang HR, Hong K, Choi H, Kang JY, Jung YW

(---)

DOI: 10.26434/chemrxiv-2024-nkftl

Related EMDB entry:

Deposited:

2024-09-21

Released:

2025-01-09

Last modified:

2025-01-09

Imageset size:

1.56 GB

Imageset DOI:

10.6019/EMPIAR-12294

Experimental metadata:

Download xml json

Contains:

reconstructed volumes

Image Sets

1. tomogram image stack of GFP(-9)-his6-mi3 emulsified LAF condensates

Category:

reconstructed volumes

Image format:

TIFF

No. of images or tilt series:

400

Image size:

(2048, 2048)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(2.4, 2.4)

Details:

tomographic reconstruction of LAF-based condensates emulsified with GFP(-9)-his6-mi3 cage proteins used as a Pickering emulsifier. thick black spots are AuNPs used as a fiducial marker.

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Citations

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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