EMPIAR-11931 - Cryo electron structure of the ectodomain of Site-one protease without SPRING [6171 multi-frame micrographs composed of 1 frames each in EER format]

EMPIAR-11931

Cryo electron structure of the ectodomain of Site-one protease without SPRING [6171 multi-frame micrographs composed of 1 frames each in EER format]

Publication:

SPRING licenses S1P-mediated cleavage of SREBP2 by displacing an inhibitory pro-domain

Hendrix S, Dartigue V, Hall H, Bawaria S, Kingma J, Bajaj B, Zelcer N, Kober DL

Nature communications 15 (2024)

PMID: 38977690

DOI: 10.1038/s41467-024-50068-8

Related EMDB entry:

EMD-42661

Deposited:

2024-03-02

Released:

2025-02-10

Last modified:

2025-02-10

Imageset size:

3.37 TB

Imageset DOI:

10.6019/EMPIAR-11931

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. eer format movies of site-one protease

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

6171

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.738, 0.738)

Details:

Unprocessed micrographs and gain ref file for data collection corresponding to EMD-42661. The pixel size was 0.738 A. The nominal dose rate was 8.5 eps and the total dose was 55 e/A2. For processing, the movies were split into 50 frames.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

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