EMPIAR-11931 - Cryo electron structure of the ectodomain of Site-one protease without SPRING [6171 multi-frame micrographs composed of 1 frames each in EER format]

EMPIAR-11931

Cryo electron structure of the ectodomain of Site-one protease without SPRING [6171 multi-frame micrographs composed of 1 frames each in EER format]

Publication:

SPRING licenses S1P-mediated cleavage of SREBP2 by displacing an inhibitory pro-domain

Hendrix S, Dartigue V, Hall H, Bawaria S, Kingma J, Bajaj B, Zelcer N, Kober DL

Nature communications 15 (2024)

PMID: 38977690

DOI: 10.1038/s41467-024-50068-8

Related EMDB entry:

EMD-42661

Deposited:

2024-03-02

Released:

2025-02-10

Last modified:

2025-03-17

Imageset size:

3.06 TB

Imageset DOI:

10.6019/EMPIAR-11931

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. eer format movies of site-one protease

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

6171

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.738, 0.738)

Details:

Unprocessed micrographs and gain ref file for data collection corresponding to EMD-42661. The pixel size was 0.738 A. The nominal dose rate was 8.5 eps and the total dose was 55 e/A2. For processing, the movies were split into 50 frames.

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Citations

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Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

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Helical reconstruction of amyloids in cryoSPARC.

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CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.

Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

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