EMPIAR-11856 - β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 5 seconds post-GTP addition [8700 multi-frame micrographs composed of 63 frames each in TIFF format]

EMPIAR-11856

β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 5 seconds post-GTP addition [8700 multi-frame micrographs composed of 63 frames each in TIFF format]

Publication:

Time-resolved cryo-EM of G-protein activation by a GPCR

Papasergi-Scott MM, Pérez-Hernández G, Batebi H, Gao Y, Eskici G, Seven AB, Panova O, Hilger D, Casiraghi M, He F, Maul L, Gmeiner P, Kobilka BK, Hildebrand PW, Skiniotis G

Nature (2024)

PMID: 38480881

DOI: 10.1038/s41586-024-07153-1

Related EMDB entry:

EMD-29991 EMD-40002 EMD-40004 EMD-42426 EMD-42422 EMD-40112 EMD-40108 EMD-40176 EMD-40174 EMD-29996 EMD-29992 EMD-40021 EMD-40020 EMD-40023 EMD-40019 EMD-40111 EMD-40172 EMD-29985 EMD-29987 EMD-29989 EMD-40005 EMD-42427 EMD-42418 EMD-40014 EMD-40096 EMD-40156 EMD-29993 EMD-40001 EMD-42425 EMD-40018 EMD-40009 EMD-40097 EMD-40103 EMD-40160 EMD-40169 EMD-29997 EMD-42414 EMD-42413 EMD-42420 EMD-42416 EMD-42410 EMD-40011 EMD-40012 EMD-40016 EMD-29994 EMD-29998 EMD-42415 EMD-42417 EMD-40114 EMD-40170 EMD-40166 EMD-40171 EMD-40157 EMD-40024 EMD-40106 EMD-42424 EMD-29986 EMD-29995 EMD-42419 EMD-42421 EMD-42411 EMD-40010 EMD-40025 EMD-40107 EMD-29988 EMD-40013 EMD-40028 EMD-40026 EMD-40104 EMD-40109 EMD-40165 EMD-40175 EMD-40168 EMD-29990 EMD-29999 EMD-40027 EMD-40022 EMD-40017 EMD-40100 EMD-40099 EMD-40158 EMD-40110 EMD-42408 EMD-42423 EMD-42409 EMD-40015 EMD-40102 EMD-40113 EMD-40159 EMD-40167 EMD-40173 EMD-40000 EMD-42412 EMD-40115 EMD-40098 EMD-40101 EMD-40105 EMD-40164 EMD-40163 EMD-40161

Deposited:

2024-01-20

Released:

2024-05-14

Last modified:

2024-05-14

Imageset size:

4.46 TB

Imageset DOI:

10.6019/EMPIAR-11856

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 5 seconds after GTP addition

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8700

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.43385, 0.43385)

Details:

Raw unaligned movie files (tif format, 63 frames, not gain corrected), and gain correction image (mrc format).
Cryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope equipped with a K3 Summit direct electron detector. Super-resolution pixel size: 0.43385 Å. Μovies were obtained at a total exposure of 60.48 electrons/Å^2.

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Citations

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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Zheng L, Xu J, Wang W, Gao X, Zhao C, Guo W, Sun L, Cheng H, Meng F, Chen B, Sun W, Jia X, Zhou X, Wu K, Liu Z, Ding F, Liu N, Wang HW, Peng H. (2024)

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