EMPIAR-11856 - β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 5 seconds post-GTP addition [8700 multi-frame micrographs composed of 63 frames each in TIFF format]

EMPIAR-11856

β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 5 seconds post-GTP addition [8700 multi-frame micrographs composed of 63 frames each in TIFF format]

Publication:

Time-resolved cryo-EM of G-protein activation by a GPCR

Papasergi-Scott MM, Pérez-Hernández G, Batebi H, Gao Y, Eskici G, Seven AB, Panova O, Hilger D, Casiraghi M, He F, Maul L, Gmeiner P, Kobilka BK, Hildebrand PW, Skiniotis G

Nature (2024)

PMID: 38480881

DOI: 10.1038/s41586-024-07153-1

Related EMDB entry:

EMD-29991 EMD-40002 EMD-40004 EMD-42426 EMD-42422 EMD-40112 EMD-40108 EMD-40176 EMD-40174 EMD-29996 EMD-29992 EMD-40021 EMD-40020 EMD-40023 EMD-40019 EMD-40111 EMD-40172 EMD-29985 EMD-29987 EMD-29989 EMD-40005 EMD-42427 EMD-42418 EMD-40014 EMD-40096 EMD-40156 EMD-29993 EMD-40001 EMD-42425 EMD-40018 EMD-40009 EMD-40097 EMD-40103 EMD-40160 EMD-40169 EMD-29997 EMD-42414 EMD-42413 EMD-42420 EMD-42416 EMD-42410 EMD-40011 EMD-40012 EMD-40016 EMD-29994 EMD-29998 EMD-42415 EMD-42417 EMD-40114 EMD-40170 EMD-40166 EMD-40171 EMD-40157 EMD-40024 EMD-40106 EMD-42424 EMD-29986 EMD-29995 EMD-42419 EMD-42421 EMD-42411 EMD-40010 EMD-40025 EMD-40107 EMD-29988 EMD-40013 EMD-40028 EMD-40026 EMD-40104 EMD-40109 EMD-40165 EMD-40175 EMD-40168 EMD-29990 EMD-29999 EMD-40027 EMD-40022 EMD-40017 EMD-40100 EMD-40099 EMD-40158 EMD-40110 EMD-42408 EMD-42423 EMD-42409 EMD-40015 EMD-40102 EMD-40113 EMD-40159 EMD-40167 EMD-40173 EMD-40000 EMD-42412 EMD-40115 EMD-40098 EMD-40101 EMD-40105 EMD-40164 EMD-40163 EMD-40161

Deposited:

2024-01-20

Released:

2024-05-14

Last modified:

2024-05-14

Imageset size:

4.46 TB

Imageset DOI:

10.6019/EMPIAR-11856

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 5 seconds after GTP addition

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8700

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.43385, 0.43385)

Details:

Raw unaligned movie files (tif format, 63 frames, not gain corrected), and gain correction image (mrc format).
Cryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope equipped with a K3 Summit direct electron detector. Super-resolution pixel size: 0.43385 Å. Μovies were obtained at a total exposure of 60.48 electrons/Å^2.

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