EMPIAR-11838
Cryo-EM of SARS-CoV-2 5' proximal stem-loops 5-6 with SL6 extended and SL5a, SL5b, and SL5c removed [16511 multi-frame micrographs composed of 40 frames each in MRC format]
Publication:

Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses

Kretsch RC, Xu L, Zheludev IN, Zhou X, Huang R, Nye G, Li S, Zhang K, Chiu W, Das R

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 38427602

Related EMDB entry:
Deposited:
2023-11-28
Released:
2024-04-07
Last modified:
2024-04-07
Imageset size:
10.76 TB
Imageset DOI:
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole_*_Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   21,641,892,592 kilobytes
Compressed size: 11,554,848,969 kilobytes
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of SARS-CoV-2 5' stem-loops 5-6 RNA.
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
16511
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.741, 0.741)
Details:
These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector with a 10eV slit of a Selectris energy filter. Over the collection, the sample was exposed evenly to 49.88electrons per angstrom squared with a total exposure time of 4.60 seconds. Desired defocuses ranged from -1 to -2 nm. The exposures are gain corrected.
Files:
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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)