EMPIAR-11838
Cryo-EM of SARS-CoV-2 5' proximal stem-loops 5-6 with SL6 extended and SL5a, SL5b, and SL5c removed [16511 multi-frame micrographs composed of 40 frames each in MRC format]
Publication:

Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses

Kretsch RC, Xu L, Zheludev IN, Zhou X, Huang R, Nye G, Li S, Zhang K, Chiu W, Das R

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 38427602

Related EMDB entry:
Deposited:
2023-11-28
Released:
2024-04-07
Last modified:
2024-04-07
Imageset size:
20.16 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of SARS-CoV-2 5' stem-loops 5-6 RNA.
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
16511
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.741, 0.741)
Details:
These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector with a 10eV slit of a Selectris energy filter. Over the collection, the sample was exposed evenly to 49.88electrons per angstrom squared with a total exposure time of 4.60 seconds. Desired defocuses ranged from -1 to -2 nm. The exposures are gain corrected.
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