EMPIAR-11754 - SPA cryo-EM micrographs of chicken ubiquitinated FANCD2-FANCI3D with ds 44-bpDNA [6515 multi-frame micrographs composed of 40 frames each in MRC format]

EMPIAR-11754

SPA cryo-EM micrographs of chicken ubiquitinated FANCD2-FANCI3D with ds 44-bpDNA [6515 multi-frame micrographs composed of 40 frames each in MRC format]

Publication:

The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination

Sijacki T, Alcón P, Chen ZA, McLaughlin SH, Shakeel S, Rappsilber J, Passmore LA

Nature structural & molecular biology 29 (2022) 881-890

PMID: 36050501

DOI: 10.1038/s41594-022-00820-9

Related EMDB entry:

EMD-15103

Deposited:

2023-10-30

Released:

2024-01-23

Last modified:

2024-01-23

Imageset size:

866.43 GB

Imageset DOI:

10.6019/EMPIAR-11754

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies in this entry (FoilHole*.mrc) were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Original size:   3,623,722,900 kilobytes
Compressed size:   908,522,525 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. multi-frame micrographs of the ubiquitinated ggD2I3D complex with 44-bp dsDNA

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

6515

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.145, 1.145)

Details:

---

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Archives and downloads the selected files into an uncompressed zip file.

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Download a list of selected files.

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Citations

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Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

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