EMPIAR-11751 - Micrographs of ER-derived vesicles from HEK293F cells [893 multi-frame micrographs composed of 8 frames each in TIFF format]

EMPIAR-11751

Micrographs of ER-derived vesicles from HEK293F cells [893 multi-frame micrographs composed of 8 frames each in TIFF format]

Publication:

Visualization of translation and protein biogenesis at the ER membrane

Gemmer M, Chaillet ML, van Loenhout J, Cuevas Arenas R, Vismpas D, Gröllers-Mulderij M, Koh FA, Albanese P, Scheltema RA, Howes SC, Kotecha A, Fedry J, Förster F

Nature 614 (2023) 160-167

PMID: 36697828

DOI: 10.1038/s41586-022-05638-5

Related EMDB entry:

EMD-15872 EMD-15879 EMD-15880 EMD-15874 EMD-15878 EMD-15890 EMD-15870 EMD-15885 EMD-15884 EMD-15886 EMD-15871 EMD-15875 EMD-15877 EMD-15873 EMD-15891 EMD-15887 EMD-15888 EMD-15876 EMD-15892

Deposited:

2023-09-20

Released:

2023-11-06

Last modified:

2023-11-06

Imageset size:

1.11 TB

Imageset DOI:

10.6019/EMPIAR-11751

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned gain-corrected micrographs of tilt-series on ER-derived vesicles from HEK293F cells

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

893

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.724, 1.724)

Details:

Micrographs of ER-derived vesicles from HEK293F cells. Tilt-series collected between +-51 degrees with a 3 degree increment (35 tilts) and cumulative dose of less than 80 e-/A^2. Each tilt was collected as a movie with 8 frames and pixel size of 1.724 A/px.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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