EMPIAR-11689
Single-particle Cryo-EM datasets of hydroxycarboxylic acid receptor signaling complexes [multiple data sets in TIFF format]
Publication:

Structural basis of hydroxycarboxylic acid receptor signaling mechanisms through ligand binding

Suzuki S, Tanaka K, Nishikawa K, Suzuki H, Oshima A, Fujiyoshi Y

Nature communications 14 (2023)

PMID: 37736747

Deposited:
2023-09-14
Released:
2023-10-03
Last modified:
2023-10-03
Imageset size:
4.64 TB
Imageset DOI:
Experimental metadata:
Download xml json
Deposited via PDBj
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame TIFF compressed movies of the HCA2-Gi complex with GSK256073
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
4872
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
Please discard the first frame, which was corrupted due to a problem with the CDS mode.
Gain reference (MRC format) is included in each folder with the movie files. No rotation or flipping is required in RELION.
The pixel size in the TIFF header is not correct.
HT: 300 kV, Cs: 2.7 mm. does per frame: 1 e/Å2.
Files:
Loading...
2. Unaligned multi-frame TIFF compressed movies of the HCA2-Gi complex with MK6892
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5806
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
Please discard the first frame, which was corrupted due to a problem with the CDS mode.
Gain reference (MRC format) is included in each folder with the movie files. No rotation or flipping is required in RELION.
The pixel size in the TIFF header is not correct.
HT: 300 kV, Cs: 2.7 mm. does per frame: 1 e/Å2
Files:
Loading...
3. Unaligned multi-frame TIFF compressed movies of the HCA2-Gi complex with LUF6283
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2828
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.99, 0.99)
Details:
Gain reference (MRC format) is included in each folder with the movie files. No rotation or flipping is required in RELION.
The number at the end of the file indicates the direction of the 3x3 image shift.
The pixel size in the TIFF header is not correct.
HT: 300 kV, Cs: 3.4 mm. does per frame: 1.74 e/Å2
Files:
Loading...
4. Unaligned multi-frame TIFF compressed movies of the HCA2-Gi complex with acifran
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3602
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.99, 0.99)
Details:
Gain reference (MRC format) is included in each folder with the movie files.
The number at the end of the file indicates the direction of the 3x3 image shift.
The pixel size in the TIFF header is not correct.
HT: 300 kV, Cs: 3.4 mm. does per frame: 1.74 e/Å2
Files:
Loading...
5. Unaligned multi-frame TIFF compressed movies of the HCA3-Gi complex with acifran
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
6440
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.78, 0.78)
Details:
Gain reference (MRC format) is included in each folder with the movie files. No rotation or flipping is required in RELION.
The number at the end of the file indicates the direction of the 3x3 image shift.
The pixel size in the TIFF header is not correct.
HT: 300 kV, Cs: 3.4 mm. does per frame: 1.78 e/Å2
Files:
Loading...
Files:
Loading...

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