EMPIAR-11663 - Single particle cryo-EM dataset of Yarrowia lipolytica mitochondrial complex I with R121M NDUFS2/NUCM mutation [2241 multi-frame micrographs composed of 40 frames each in MRC format]

EMPIAR-11663

Single particle cryo-EM dataset of Yarrowia lipolytica mitochondrial complex I with R121M NDUFS2/NUCM mutation [2241 multi-frame micrographs composed of 40 frames each in MRC format]

Publication:

A conserved arginine residue is critical for stabilizing the N2 FeS cluster in mitochondrial complex I

Hameedi MA, Grba DN, Richardson KH, Jones AJY, Song W, Roessler MM, Wright JJ, Hirst J

The Journal of biological chemistry 296 (2021)

PMID: 33640456

DOI: 10.1016/j.jbc.2021.100474

Related EMDB entry:

EMD-11969

Deposited:

2023-08-02

Released:

2024-06-27

Last modified:

2024-06-27

Imageset size:

505.60 GB

Imageset DOI:

10.6019/EMPIAR-11663

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (FoilHole*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply the gain reference (gain-reference.mrc) during processing.

Original size:   4,985,870,926 kilobytes
Compressed size:   530,160,611 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of NUCM R121M Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2241

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.07, 1.07)

Details:

Unaligned multi-frame micrographs of NUCM R121M Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM. Frozen gold grids treated with PEGylated thiol reagent. Images (MRC) were collected with a 300 keV FEI Titan Krios on a Gatan K2 Summit detector, using counting mode and a Gatan BioContinuum energy filter (20 eV). 10 s exposures were captured in 40 frames with a total dose of ~48 e/Å-2. A nominal pixel size of 1.07 Å/pixel was used (calibrated 1.05 Å/pixel using pre-existing models).

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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