EMPIAR-11663
Single particle cryo-EM dataset of Yarrowia lipolytica mitochondrial complex I with R121M NDUFS2/NUCM mutation [2241 multi-frame micrographs composed of 40 frames each in MRC format]
Publication:

A conserved arginine residue is critical for stabilizing the N2 FeS cluster in mitochondrial complex I

Hameedi MA, Grba DN, Richardson KH, Jones AJY, Song W, Roessler MM, Wright JJ, Hirst J

The Journal of biological chemistry 296 (2021)

PMID: 33640456

Related EMDB entry:
Deposited:
2023-08-02
Released:
2024-06-27
Last modified:
2024-06-27
Imageset size:
505.60 GB
Imageset DOI:
Experimental metadata:
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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (FoilHole*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply the gain reference (gain-reference.mrc) during processing.

Original size:   4,985,870,926 kilobytes
Compressed size:   530,160,611 kilobytes
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of NUCM R121M Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM.
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
2241
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.07, 1.07)
Details:
Unaligned multi-frame micrographs of NUCM R121M Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM. Frozen gold grids treated with PEGylated thiol reagent. Images (MRC) were collected with a 300 keV FEI Titan Krios on a Gatan K2 Summit detector, using counting mode and a Gatan BioContinuum energy filter (20 eV). 10 s exposures were captured in 40 frames with a total dose of ~48 e/Å-2. A nominal pixel size of 1.07 Å/pixel was used (calibrated 1.05 Å/pixel using pre-existing models).
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