EMPIAR-11663 - Single particle cryo-EM dataset of Yarrowia lipolytica mitochondrial complex I with R121M NDUFS2/NUCM mutation [2241 multi-frame micrographs composed of 40 frames each in MRC format]

EMPIAR-11663

Single particle cryo-EM dataset of Yarrowia lipolytica mitochondrial complex I with R121M NDUFS2/NUCM mutation [2241 multi-frame micrographs composed of 40 frames each in MRC format]

Publication:

A conserved arginine residue is critical for stabilizing the N2 FeS cluster in mitochondrial complex I

Hameedi MA, Grba DN, Richardson KH, Jones AJY, Song W, Roessler MM, Wright JJ, Hirst J

The Journal of biological chemistry 296 (2021)

PMID: 33640456

DOI: 10.1016/j.jbc.2021.100474

Related EMDB entry:

EMD-11969

Deposited:

2023-08-02

Released:

2024-06-27

Last modified:

2024-06-27

Imageset size:

505.60 GB

Imageset DOI:

10.6019/EMPIAR-11663

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

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Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of NUCM R121M Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2241

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.07, 1.07)

Details:

Unaligned multi-frame micrographs of NUCM R121M Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM. Frozen gold grids treated with PEGylated thiol reagent. Images (MRC) were collected with a 300 keV FEI Titan Krios on a Gatan K2 Summit detector, using counting mode and a Gatan BioContinuum energy filter (20 eV). 10 s exposures were captured in 40 frames with a total dose of ~48 e/Å-2. A nominal pixel size of 1.07 Å/pixel was used (calibrated 1.05 Å/pixel using pre-existing models).

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Citations

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Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)