EMPIAR-11596
Seeded Aβ(1-40) amyloid fibril [3018 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:

Cryo-EM analysis of the effect of seeding with brain-derived Aβ amyloid fibrils

Pfeiffer PB, Ugrina M, Schwierz N, Sigurdson CJ, Schmidt M, Fändrich M

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Related EMDB entry:
Deposited:
2023-05-31
Released:
2024-03-18
Last modified:
2024-03-18
Imageset size:
550.18 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of seeded Aβ(1-40) amyloid fibrils
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3018
Image size:
(3838, 3710)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.04, 1.04)
Details:
Krios1 Quantum-K2
Cs 2.7
300kV EFTEM nanpprobe
x130k spot 8 beam size 650nm
c2=50 objective_aperture=100

GIF-K2 counting
10s exposure 40 frames
total dose at the sample 53.79e/A2
1 pixel = 1.04A
dose rate ~4-5 e/pix/s

defocus range -0.8 to -2.0

The gain reference (/data/data/CountRef_map_0117_001.dm4) is not applied to the micrographs. The NOTES.txt file contains EM information. Thumbnails and power spectra (/data/thumbs) as well as coordinates (/data/manual pick) are provided.
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