EMPIAR-11487 - Single particle cryo-EM dataset of extended cowpea chlorotic mottle virus in plunge-frozen vitrified ice [multiple data sets in EER format]

EMPIAR-11487

Single particle cryo-EM dataset of extended cowpea chlorotic mottle virus in plunge-frozen vitrified ice [multiple data sets in EER format]

Publication:

Fast viral dynamics revealed by microsecond time-resolved cryo-EM

Harder OF, Barrass SV, Drabbels M, Lorenz UJ

Nature communications 14 (2023)

PMID: 37704664

DOI: 10.1038/s41467-023-41444-x

Related EMDB entry:

EMD-16790

Deposited:

2023-03-01

Released:

2023-11-07

Last modified:

2023-11-07

Imageset size:

5.11 TB

Imageset DOI:

10.6019/EMPIAR-11487

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Single particle cryo-EM dataset of extended cowpea chlorotic mottle virus in plunge-frozen vitrified ice, grid 1

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

5391

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.726, 0.726)

Details:

These are the EER files from Falcon 4 detector - raw micrographs, not motion corrected.

Samples of expanded CCMV are prepared at pH 7.6 in the absence of divalent ions and plunge frozen in the presence of a photoacid (NPE-caged proton). The pH of the sample is then lowered to 4.5 by releasing the photoacid with UV irradiation (266 nm).

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2. Single particle cryo-EM dataset of extended cowpea chlorotic mottle virus in plunge-frozen vitrified ice, grid 2

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

5235

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.726, 0.726)

Details:

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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