The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.
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The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.
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The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.
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The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.
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Merged set of pre-60S particles from four datasets. Particles are polished, magnification anisotropy corrected and per-particle defocus corrected. A Relion .star format file is provided and contains the final Euler angles, shifts and also the micrograph coordinates.
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